EisnerAmper’s industry groups include public companies; technology, information, media, entertainment, and sports (which the firm calls TIMES); financial services; real estate; law firms; not-for-profit organizations; and health sciences. Past and present clients include some big names such as the National Football League’s New York Jets, Manhattan-based charity God’s Love We Deliver, private equity superstars Apax Partners, Major League Soccer, and the Aaron Diamond AIDS Research Center.

Service lines include a full range of accounting, audit, and tax solutions, as well as specialized consulting and advisory services. Asset management is offered through the firm’s TAG Eisner arm, which serves families and wealthy individuals; Eisner Information Solutions and Eisner Resource Staffing are also separate units that offer technology services and recruitment services, respectively. The firm's Eisner Fund Services works with hedge funds, private equity funds, and family offices.

The firm might be based in New York City, but its work spans the globe: for many decades the firm has worked with multinational clients who do business in the U.S., helping them structure, organize, and manage their business and reporting requirements. EisnerAmper’s cross border practice has evolved into a full lifecycle operation. The firm provides startup services like advising on corporate structures; assisting in obtaining U.S. federal identification numbers; advising on U.S. business practices and preparing mountains of paperwork; ongoing operations services, including record keeping and reporting, payroll, and training; advising on U.S. insurance and reporting requirements and staffing; and tax planning and compliance services, including return preparation, transfer pricing, tax structure coordination and advising on the tax implications when foreign nationals work in the U.S.